This research developed a protocol that simulates invasive cervical resorption (ICR) in human teeth, diagnostic by periapical radiography and cone beam CT (CBCT), to be reproducible and applicable in studies evaluating diagnostic imaging methods such resorption. Sixteen healthy human teeth were divided into four groups. These teeth were sectioned along its long axis and irregular cavities were created with diamond FG reproducing the ICRs classes 1, 2, 3 and 4 proposed by Heithersay. After bonding of longitudinal segments, the teeth were evaluated by means of digital periapical radiography (DPR) and Cone Beam CT (CBCT). The images obtained were compared with images of some clinical cases of ICR. Images obtained by DR and CBCT were similar to those described by Heithersay, as well as images of clinical cases. The in vitro ICRs held by the proposed method are reproducible and represent the radiographic and tomographic features of these lesions.