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Methyl mercury-induced combined inhibition of ATP regeneration and protein synthesis in reticulocyte lysate cell-free translation system.

Authors
  • Kuznetsov, D A
  • Zavijalov, N V
  • Govorkov, A V
  • Ivanov-Snaryad, A A
Type
Published Article
Journal
Toxicology Letters
Publisher
Elsevier
Publication Date
Mar 01, 1986
Volume
30
Issue
3
Pages
267–271
Identifiers
PMID: 3705108
Source
Medline
License
Unknown

Abstract

Methyl mercury inhibits in vitro protein synthesis in the rabbit reticulocyte lysate cell-free translation system and simultaneously leads to reduction of the ATP/ADP index. It has been established that there is a close relationship (r = 0.86) between the rates of ATP resynthesis and protein synthesis in vitro within a wide range of the methyl mercury concentrations tested (0.0001-1.0 mumol/ml). Ammonia, CCl4, cycloheximide and pactamycin inhibit translation in vitro without affecting ATP resynthesis. Methanol does not cause substantial alterations of the parameters of the cell-free translation system. Thus, there are at least two essentially different causes of poison-induced in vitro translation blocking. Suppression of ATP resynthesis (methyl mercury) and direct effect on protein synthesis (cycloheximide, CCl4, etc.)

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