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Methods for transient assay of gene function in floral tissues.

Authors
  • Shang, Yongjin
  • Schwinn, Kathy E
  • Bennett, Michael J
  • Hunter, Donald A
  • Waugh, Toni L
  • Pathirana, Nilangani N
  • Brummell, David A
  • Jameson, Paula E
  • Davies, Kevin M
Type
Published Article
Journal
Plant Methods
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 01, 2007
Volume
3
Pages
1–1
Identifiers
PMID: 17207290
Source
Medline
License
Unknown

Abstract

We demonstrate the feasibility of using biolistics-based transient RNAi, and transient transformation of petal tissue via Agrobacterium infiltration to study gene function in petals. We have also produced a vector for high throughput gene silencing studies, incorporating the option of using T-A cloning to insert the gene sequence of interest. These techniques should allow analysis of gene function in a much broader range of flower species.

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