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Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system.

Authors
  • Sawasaki, Tatsuya
  • Morishita, Ryo
  • Gouda, Mudeppa D
  • Endo, Yaeta
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2007
Volume
375
Pages
95–106
Identifiers
PMID: 17634598
Source
Medline
License
Unknown

Abstract

Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.

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