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Methods for detection of GMOs in food and feed.

Authors
  • Marmiroli, Nelson
  • Maestri, Elena
  • Gullì, Mariolina
  • Malcevschi, Alessio
  • Peano, Clelia
  • Bordoni, Roberta
  • De Bellis, Gianluca
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
Oct 01, 2008
Volume
392
Issue
3
Pages
369–384
Identifiers
DOI: 10.1007/s00216-008-2303-6
PMID: 18726090
Source
Medline
License
Unknown

Abstract

This paper reviews aspects relevant to detection and quantification of genetically modified (GM) material within the feed/food chain. The GM crop regulatory framework at the international level is evaluated with reference to traceability and labelling. Current analytical methods for the detection, identification, and quantification of transgenic DNA in food and feed are reviewed. These methods include quantitative real-time PCR, multiplex PCR, and multiplex real-time PCR. Particular attention is paid to methods able to identify multiple GM events in a single reaction and to the development of microdevices and microsensors, though they have not been fully validated for application.

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