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Methodology to label mixed carbohydrate components by APTS.

Authors
  • Bui, Annamária1
  • Kocsis, Béla
  • Kilár, Ferenc
  • 1 Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary. , (Hungary)
Type
Published Article
Journal
Journal of Biochemical and Biophysical Methods
Publisher
Elsevier
Publication Date
Apr 24, 2008
Volume
70
Issue
6
Pages
1313–1316
Identifiers
DOI: 10.1016/j.jprot.2008.01.009
PMID: 18316129
Source
Medline
License
Unknown

Abstract

Labelling of carbohydrates with fluorescent dyes is of importance in the analysis of complex oligo- or polysaccharides. The complexity of the hydrolization mixtures of the oligosaccharides can be studied with separation methods after labelling. However, the procedures might provide unexpected phenomena. The experimental circumstances for labelling carbohydrates with 8-aminopyrene-1,3,6-trisulfonic acid were studied and the labelling efficiency of mono and oligosaccharides present in endotoxins was followed by capillary electrophoresis using LIF detection. Significant differences were observed in the labelling of the different sugar molecules, and the lowest reactivity with the dye was observed in the cases of amino-sugars. The results provide a basis for studies in determining the structures of the core parts and the O-chains of bacterial endotoxins.

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