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Method for Testing Degree of Infectivity of Rhizobium meliloti Strains.

Authors
  • Olivares, J
  • Casadesús, J
  • Bedmar, E J
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
May 01, 1980
Volume
39
Issue
5
Pages
967–970
Identifiers
PMID: 16345574
Source
Medline
License
Unknown

Abstract

The infectiveness of different strains of Rhizobium meliloti was tested with a technique that uses the addition of tetracycline to the root medium. To stop the infection, the antibiotic was added some time after the inoculation of Medicago sativa plants. A coefficient of infectivity for each strain was calculated according to the number of nodules that appeared with and without the addition of the antibiotic. This method seems useful in infectivity studies and is simpler and easier to perform than the test of competence between strains.

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