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A method for selection of mutations at the tdk locus in Escherichia coli.

Authors
Type
Published Article
Journal
Journal of Bacteriology
0021-9193
Publisher
American Society for Microbiology
Publication Date
Volume
175
Issue
18
Pages
6049–6051
Identifiers
PMID: 8376351
Source
Medline

Abstract

Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk.

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