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Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier.

Authors
  • Li, Ting1
  • Wang, Wenyan2
  • Cheng, Yingying3
  • Han, Wenling4
  • 1 Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, China; Peking University Center for Human Disease Genomics, Beijing, 100191, China. , (China)
  • 2 Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, China; Peking University Center for Human Disease Genomics, Beijing, 100191, China; Tsinghua University School of Medicine, Beijing 100084, China. , (China)
  • 3 Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, China; Department of Laboratory Medicine, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China. , (China)
  • 4 Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, China; Peking University Center for Human Disease Genomics, Beijing, 100191, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of Bioscience and Bioengineering
Publisher
Elsevier
Publication Date
Mar 01, 2018
Volume
125
Issue
3
Pages
359–364
Identifiers
DOI: 10.1016/j.jbiosc.2017.10.008
PMID: 29167066
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

FAM19A4 (family with sequence similarity 19 member A4; also TAFA4) is a classical secretory protein expressed mainly in the central nervous system and upregulated significantly in lipopolysaccharide (LPS)-stimulated monocytes and macrophages. It is a novel cytokine ligand of formyl peptide receptor 1 (FPR1), showing chemotactic activities on macrophages and promoting the phagocytosis capacity and the release of reactive oxygen species (ROS) by macrophages upon zymosan stimulation. Based on the same detection principle as enzyme-linked immunosorbent assay (ELISA), we developed a sandwich immunoassay for quantitative detection of FAM19A4 in biological fluids by flow cytometry, with latex beads as solid carrier. The method showed good performance in a wide range of 39-10,000 pg/mL and possessed excellent specificity, good precision, and favorable recovery in several different matrices. Native FAM19A4 secreted by phorblo 12-myristate 13-acetate (PMA) and LPS stimulated THP-1 cells could also be detected by this method. This method will be much helpful to FAM19A4 studies. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

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