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[Method for the photometric determination of aldehyde oxidase activity].

Authors
Type
Published Article
Journal
Biomedica biochimica acta
Publication Date
Volume
43
Issue
11
Pages
1223–1226
Identifiers
PMID: 6532455
Source
Medline
License
Unknown

Abstract

A method has been developed for the determination of aldehyde oxidase. Dimethylaminocinnamaldehyde was taken as substrate. It is oxidized to the corresponding acid and changes ist absorption at 398 nm. Except dissolved oxygen no additional electron acceptor is needed. This alternative assay was found to be about twice as sensitive compared to the method with acetaldehyde and dichlorophenol indophenol. Aldehyde oxidase activity was estimated in the raw extract of livers of pig, sheep and rat. Xanthine oxidase does not interfere.

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