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A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.

Authors
  • Russell, S D
Type
Published Article
Journal
Stain technology
Publication Date
Jan 01, 1990
Volume
65
Issue
5
Pages
259–261
Identifiers
PMID: 2278052
Source
Medline
License
Unknown

Abstract

A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.

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