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A Method to Estimate Acetylcholinesterase-Active Sites and Turnover in Insects

Authors
  • Charpentier, Alice
  • Menozzi, Philippe
  • Marcel, Véronique
  • Villatte, François
  • Fournier, Didier
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 2000
Volume
285
Issue
1
Pages
76–81
Identifiers
DOI: 10.1006/abio.2000.4738
Source
Elsevier
Keywords
License
Unknown

Abstract

Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific.

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