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[Method of contrast enhancement of DNA-containing structures on ultrathin sections].

Authors
Type
Published Article
Journal
Biulleten' eksperimental'noĭ biologii i meditsiny
Publication Date
Volume
89
Issue
6
Pages
747–748
Identifiers
PMID: 7397370
Source
Medline
License
Unknown

Abstract

Thin epoxy sections of formaldehyde-fixed tissue underwent depurinizing hydrolysis with 5N HCl at room temperature for 1 hour, the treatment with an aldehyde blocking agent, 10% sodium bisulfite, at 60 degrees C during 90 min, and staining with 2% aqueous uranyl acetate. Introduction of bisulfite considerably enhanced the contrast of chromatin. Staining of DNA in these conditions is summed up of preferential binding of uranyl cations with phosphates of DNA and with bisulfite anions specifically bound to deoxyribose.

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