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Method for bacteriophage isolation against target Campylobacter strains.

Authors
Type
Published Article
Journal
Letters in applied microbiology
Publication Date
Volume
50
Issue
2
Pages
192–197
Identifiers
DOI: 10.1111/j.1472-765X.2009.02774.x
PMID: 20002571
Source
Medline
License
Unknown

Abstract

This technique will be valuable in the context of phage therapy for enriching for phages that are active against specifically identified strains of bacteria, for example from a food poisoning outbreak or epidemic strains resistant to multiple antibiotics. In these situations, using the conventional methods for searching for bacteriophages active for these particular strains can be a time-consuming, if not an unsuccessful process. Using the isolation method described in this manuscript, the particular strains can be added to the enrichment broth increasing the probability of finding phages against them. Therefore, it will shorten the time needed for seeking phages able to lyse target strains, which in most of the cases, because of the rapid increase in antimicrobial-resistant bacteria, is of crucial importance.

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