Pavetta indica L. is used in traditional medicine for the treatment of various diseases including hemorrhoids, headache, urinary conditions, ulcerated nose, and dropsy. However, no study has evaluated the anticancer effect of P. indica L. In this study, we found that a methanol extract of the leaves and branches of P. indica L. (MEPI) caused cellcycle arrest at the subG1 phase and induced apoptosis, as indicated by the activation of caspase8, 3, 7, and cPARP. Western blotting revealed that MEPI significantly reduced the levels of markers of the epithelialmesenchymal transition, such as Vimentin, Snail, Slug, and matrix metallopeptidase 9. Notably, the expression of multidrug resistanceassociated protein 1 in triple negative breast cancer (TNBC) was significantly decreased by MEPI. Moreover, the cotreatment with MEPI and doxorubicin resulted in a synergistic reduction in cell viability. MEPI also induced radiation sensitization of TNBC cells. Gas chromatographymass spectrometry analysis revealed that 5,6dehydrokawain (DK) is the major constituent of MEPI. Interestingly, DK exerted significant antiinvasive and antimetastatic effects. Our results provide a strong rationale for investigating the molecular mechanisms of action of MEPI in TNBC.