Affordable Access

Metallothionein cDNA, promoter, and genomic sequences of the tropical green mussel, Perna viridis.

Authors
  • Khoo, H W
  • Patel, K H
Type
Published Article
Journal
The Journal of experimental zoology
Publication Date
Sep 01, 1999
Volume
284
Issue
4
Pages
445–453
Identifiers
PMID: 10451422
Source
Medline
License
Unknown

Abstract

The primary structure of the cDNA and metallothionein (MT) genomic sequences of the tropical green mussel (Perna viridis) was determined. The complete cDNA sequences were obtained using degenerate primers designed from known metallothionein consensus amino acid sequences from the temperate species Mytilus edulis. The amino acid sequences of P. viridis metallothionein deduced from the coding region consisted of 72 amino acids with 21 cysteine residues and 9 Cys-X-Cys motifs corresponding to Type I MT class of other species. Two different genomic sequences coding for the same mRNA were obtained. Each putative gene contained a unique 5'UTR and two unique introns located at the same splice sites. The promoters for both genes were different in length and both contained metal responsive elements and active protein-binding sites. The structures of the genomic clones were compared with those of other species. J. Exp. Zool. 284:445-453, 1999.

Report this publication

Statistics

Seen <100 times