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Metagenomic Deep Sequencing to Investigate for an Infectious Etiology of Iridocorneal Endothelial Syndrome.

Authors
  • Sutra, Plern1, 2, 3
  • Rose-Nussbaumer, Jennifer1, 2
  • Gonzales, John A1, 2
  • Wang, Kaidi1, 2
  • Hinterwirth, Armin1
  • Seitzman, Gerami1, 2
  • Bloomer, Michele2
  • Acharya, Nisha1, 2
  • Doan, Thuy1, 2
  • 1 Francis I. Proctor Foundation, University of California, San Francisco, San Francisco, CA.
  • 2 Department of Ophthalmology, University of California, San Francisco, San Francisco, CA.
  • 3 Department of Ophthalmology, Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand. , (Thailand)
Type
Published Article
Journal
Cornea
Publication Date
Oct 01, 2020
Volume
39
Issue
10
Pages
1307–1310
Identifiers
DOI: 10.1097/ICO.0000000000002368
PMID: 32398422
Source
Medline
Language
English
License
Unknown

Abstract

Iridocorneal endothelial (ICE) syndrome is a group of rare ocular conditions that result from abnormal corneal endothelial cells, leading to secondary glaucoma, iris distortions, and corneal edema. The etiology of ICE is unknown, although it has been associated with viral infections, such as herpes simplex virus. In this study, we sought to identify an infectious etiology for ICE using advanced molecular techniques. Metagenomic RNA sequencing (MDS) is a high-throughput sequencing approach that can identify all pathogens in any clinical sample, including RNA viruses. Descemet membrane and aqueous fluid from patients with ICE syndrome were subjected to MDS testing. Samples from 3 patients with ICE were analyzed. MDS was performed on the aqueous fluid of 3 patients and Descemet membrane and endothelial cell tissue from 1 patient. Viral pathogens were not identified in any of the samples. We were unable to identify a viral etiology in the tissues of patients with the Chandler variant of ICE syndrome, although this study was limited by sample size.

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