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Metabolites of Lactobacillus plantarum 2142 Prevent Oxidative Stress-Induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

Authors
  • Paszti-Gere, Erzsebet1
  • Szeker, Krisztina1
  • Csibrik-Nemeth, Edina1
  • Csizinszky, Rita1
  • Marosi, Andras1
  • Palocz, Orsolya1
  • Farkas, Orsolya1
  • Galfi, Peter1
  • 1 Szent István University, Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences, István u. 2, Budapest, 1078, Hungary , Budapest (Hungary)
Type
Published Article
Journal
Inflammation
Publisher
Springer-Verlag
Publication Date
Apr 04, 2012
Volume
35
Issue
4
Pages
1487–1499
Identifiers
DOI: 10.1007/s10753-012-9462-5
Source
Springer Nature
Keywords
License
Yellow

Abstract

Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunmodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-α level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, d- and l-lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.

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