Affordable Access

Metabolism of quercetin and kaempferol by rat hepatocytes and the identification of flavonoid glycosides in human plasma.

Authors
  • Oliveira, E J
  • Watson, D G
  • Grant, M H
Type
Published Article
Journal
Xenobiotica; the fate of foreign compounds in biological systems
Publication Date
Apr 01, 2002
Volume
32
Issue
4
Pages
279–287
Identifiers
PMID: 12028662
Source
Medline
License
Unknown

Abstract

1. The metabolism of the flavonoids quercetin and kaempferol by rat hepatocytes was investigated using liquid chromatography coupled with electrospray mass spectrometry (LC-ESI MS). Quercetin and kaempferol were extensively metabolized (98.8 +/- 0.1% and 81.0 +/- 5.1% respectively, n = 4), with four glucuronides of quercetin and two of kaempferol being detected after incubation. 2. The glucuronides of quercetin and kaempferol formed upon incubation with rat hepatocytes were identified as the same ones formed after incubation with the UDP-glucuronosyltransferase isoform UGT1A9. 3. In addition, plasma samples from human volunteers taken after consumption of capsules of Ginkgo biloba, a plant rich in flavonoid glycosides, were analysed by LC-MS for the presence of flavonoid glucuronides and flavonoid glycosides. Reported is evidence for the presence of flavonoid glycosides in samples of plasma. 4. The results suggest that UGT1A9 is a key UDP-glucuronosyltransferase isoform for the metabolism of flavonoids, and that absorption of intact flavonoid glycosides is possible.

Report this publication

Statistics

Seen <100 times