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Metabolism of 2-acetylaminofluorene in cultured human lymphocytes.

Authors
  • McManus, M E
  • Trainor, K J
  • Morley, A A
  • Burgess, W
  • Stupans, I
  • Birkett, D J
Type
Published Article
Journal
Research communications in chemical pathology and pharmacology
Publication Date
Mar 01, 1987
Volume
55
Issue
3
Pages
409–418
Identifiers
PMID: 3575877
Source
Medline
License
Unknown

Abstract

The capacity of lymphocytes from 23 human subjects to metabolize the model carcinogen 2-acetylaminofluorene (AAF) was assessed. These cells metabolized AAF to its 1-, 7- and N-hydroxylated metabolites. Alpha-naphthoflavone and metyrapone exhibited IC50's for all pathways of 0.02 microM and 1 mM, respectively. These data indicate that the same form of cytochrome P450 or forms with similar IC50's are mediating these reactions. No significant difference was observed between lymphocytes from smokers versus non-smokers in the N-hydroxylation of AAF, the initial step in the metabolic activation of this carcinogen. However, lymphocytes from smokers were faster detoxifiers of AAF as seen in their increased capacity to 1- and 7-hydroxylate AAF compared to lymphocytes from non-smokers.

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