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Metabolism of [1- 14c]palmitic acid in the developing brain:Persistence of radioactivity in the carboxyl carbon

Authors
  • Dhopeshwarkar, G.A.
  • Subramanian, Carole
  • Mead, James F.
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Publisher
Elsevier
Publication Date
Jan 01, 1973
Volume
296
Issue
2
Pages
257–264
Identifiers
DOI: 10.1016/0005-2760(73)90084-2
Source
Elsevier
Keywords
License
Unknown

Abstract

[1- 14C]Palmitic acid was injected intraperitoneally into young rats (12–13 daysold) to study the label distribution over an extended period of 2 months during whichtime rapid growth and active myelination occurs. It was found that, 24 h after injection,brain palmitate still had 78% relative carboxyl activity. It was also noted thatlabel persisted in the carboxyl carbon of brain palmitate even after an extended periodof 2 months. Radioactivity distribution and specific activities of 18:0, 18:1, 20:4 and 22:6,conclusively showed that these fatty acids were synthesized by chain elongation.Similarly, radioactivity in the cholesterol fraction indicated that injected palmitatewas degraded to form radioactive acetate; thus it was surprising to find that radioactivityin brain palmitate was not redistributed by de novo synthesis from radioactiveacetate. Hypothetical possibilities for this finding are discussed.

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