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Metabolic profiling of rodent biological fluids via 1H NMR spectroscopy using a 1 mm microlitre probe.

Authors
Type
Published Article
Journal
The Analyst
Publication Date
Volume
127
Issue
5
Pages
582–584
Identifiers
PMID: 12081031
Source
Medline

Abstract

The application of a 1 mm TXI (1H/13C/15N) microlitre NMR probe with z-gradient for metabolic profiling of biofluids is described. The probe was used to provide spectral profiles for rat blood plasma using only approximately 2 microl of fluid with a range of solvent suppression techniques. Using a similar amount of fluid, spectra were obtained from rat and mouse cerebrospinal fluid, demonstrating that the probe could be used to profile rodents metabolically via biofluids previously inaccessible to NMR analysis without the need for termination.

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