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Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of MethylketonesW⃞

Authors
  • Eyal Fridman
  • Jihong Wang
  • Yoko Iijima
  • John E. Froehlich
  • David R. Gang
  • John Ohlrogge
  • Eran Pichersky
Publisher
American Society of Plant Biologists
Publication Date
Apr 01, 2005
Source
PMC
Keywords
Disciplines
  • Biology
  • Chemistry
License
Unknown

Abstract

Metabolic, Genomic, and Biochemical Analyses of Glandular Trichomes from the Wild Tomato Species Lycopersicon hirsutum Identify a Key Enzyme in the Biosynthesis of Methylketones by Eyal Fridman, Jihong Wang, Yoko Iijima, John E. Froehlich, David R. Gang, John Ohlrogge, and Eran Pichersky Plantcell Volume 17(4):1252-1267 April 1, 2005 ©2005 by American Society of Plant Biologists Structures of Common Methylketones Found in Plants. Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists Glandular Trichomes of Tomato.(A) A light microscope picture of the adaxial surface of a young leaf, showing the predominant four-celled glandular trichomes (in focus) and the less abundant long, nonglandular trichomes.(B) Scanning electron micrograph of th... Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists Localization of Methylketones in Glandular Trichomes of Tomato.(A) Relative levels of methylketone content in different tissues of the plant, per unit fresh weight. Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists In Silico Analysis of the L. hirsutum Leaf Glandular Trichome EST Databases.(A) Analysis of the enzymes of the fatty acid biosynthesis pathway. Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists Amino Acid Sequence Alignment Comparing the L. hirsutum f glabratum Accession PI126449 MKS1 Protein with Two Functionally Characterized Esterases Belonging to the α/β-Hydrolase Superfamily.The MKS1 accession number is AY701574. Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists Correlation between MKS1 Transcript and Protein and Methylketone Content in Leaves and Glands of Different Tomato Accessions.(A) RNA gel blots of MKS1 mRNA abundance in young leaves and in glands of various Lycopersicon accessions. Fridman E et al. Plantcell 2005;17:1252-1267 ©2005 by American Society of Plant Biologists

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