A sizeable number of studies have documented a relationship between heterosexual persons' experience of disgust (measured as an individual difference variable or induced experimentally) and prejudice toward gay men (i.e., homonegativity). Yet, to date, no one has attempted to meta-analytically review this corpus of research. We address this gap by conducting a meta-analysis of published and unpublished work examining heterosexual men and women's disgust and their homonegativity toward gay men. Fourteen articles (12 published, two unpublished) containing 17 studies were analyzed (N = 7,322). The average effect size for disgust sensitivity studies was moderate to large (d = 0.64), whereas for disgust induction studies, the effect was large (d = 0.77). No evidence of effect size heterogeneity emerged. Future directions and recommendations for methodological improvements are outlined.