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MESIA: Magnetic force-assisted electrochemical sandwich immunoassays for quantification of prostate-specific antigen in human serum.

Authors
  • Hwang, Hyundoo1
  • Choi, Eunjong2
  • Han, Seungmok2
  • Lee, Yunsung2
  • Choi, Taehwa2
  • Kim, Mikyoung2
  • Shin, Haegong2
  • Kim, Jaesik2
  • Choi, Jaekyu2
  • 1 BBB Inc., 26 Samseong-ro 85-gil, Gangnam-gu, Seoul, 06194, Republic of Korea. Electronic address: [email protected] , (North Korea)
  • 2 BBB Inc., 26 Samseong-ro 85-gil, Gangnam-gu, Seoul, 06194, Republic of Korea. , (North Korea)
Type
Published Article
Journal
Analytica chimica acta
Publication Date
Jul 11, 2019
Volume
1061
Pages
92–100
Identifiers
DOI: 10.1016/j.aca.2019.02.018
PMID: 30926043
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We propose a new immunoassay technique, called magnetic-force assisted electrochemical sandwich immunoassay (MESIA), where serum biomarkers can be determined by magnetic actuation and electrochemical detection of gold-coated iron oxide nanoparticles as probes for immunocomplex formation. In MESIA, neither washing buffer nor fluidic parts are necessary, because the formation of immunocomplexes and the removal of unbound probes are controlled by magnetic forces. Electrochemical pretreatment and measurement of the gold-coated magnetic probes allows highly sensitive, precise, and robust system for quantification of target analytes. Using MESIA, the concentration of prostate-specific antigen (PSA) in 10 μl of human serum is determined within 5 min. The limit of detection is 0.085 ng/mL, and the average coefficient of variance is 8.85% for five different PSA concentrations ranging from 0 to 25 ng/mL. This method shows good precision and reproducibility (<10%) and high correlation with cobas e 801 (r = 0.997) for clinical patient samples. We believe this technique to be useful in the development of a point-of-care testing platform for diagnosis and prognosis of various diseases, such as cancer, based on quantification of biomarkers in a drop of blood. Copyright © 2019 Elsevier B.V. All rights reserved.

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