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(-)-Menthol inhibits DNA topoisomerases I, II alpha and beta and promotes NF-kappaB expression in human gastric cancer SNU-5 cells.

Authors
  • Lin, Jing-Pin
  • Lu, Hsu-Feng
  • Lee, Jau-Hong
  • Lin, Jaung-Gung
  • Hsia, Te-Chun
  • Wu, Lii-Tzu
  • Chung, Jing-Gung
Type
Published Article
Journal
Anticancer research
Publication Date
Jan 01, 2005
Volume
25
Issue
3B
Pages
2069–2074
Identifiers
PMID: 16158947
Source
Medline
License
Unknown

Abstract

It has been reported that (-)-Menthol can inhibit the growth of rat liver epithelial tumor cells and is a potent chemopreventive agent. The purpose of the present experiment was to examine and identify cellular processes leading to cell death which are affected by (-)-Menthol in human gastric SNU-5 cancer cells. Cell death (cytotoxicity) was examined and analyzed by trypan blue stain and flow cytometric methods. It was shown that (-)-Menthol inhibited the proliferation of the cells in a dose- and time-dependent manner, inhibited topoisomerase I, IIa and IIbeta, but promoted the levels of NF-kappaB gene expression based on the Western blot and polymerase chain reaction (PCR) and cDNA microarray methods. These data suggest that (-)-Menthol may induce cytotoxicity through inhibiting gene expression of topoisomerase I, IIalpha and IIbeta and promoting the gene expression of NF-kappaB in SNU-5 cells.

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