The mental health of nurses was examined 2 years follow-up using the Japanese version of the 60-item General Health Questionnaire (GHQ). A total of 40 nurses in our hospital responded to the GHQ. There was a significant correlation between the GHQ scores of the first year and two years later in all nurses (p = 0.0001). There were no changes of GHQ score in two years in all nurses. However, nurses who worked on surgical ward had significantly higher scores than those who worked in internal medicine and those assigned to the elderly medicine ward in two years later GHQ score, especially anxiety and sleep disturbance, social dysfunction, and depression (p < 0.05). In the percentage of scores over 17 was no significant difference in three wards and also in the percentage of symptomatic subjects was no significant difference in three wards. Our results suggest that the mental health of nurses who worked on surgical ward might suffer more than that of those who worked in internal medicine and those assigned to the elderly medicine and we must find methods developed to improve it.