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Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer.

Authors
  • Knowles, Timothy J
  • Finka, Rachael
  • Smith, Corinne
  • Lin, Yu-Pin
  • Dafforn, Tim
  • Overduin, Michael
Type
Published Article
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Publication Date
Jun 10, 2009
Volume
131
Issue
22
Pages
7484–7485
Identifiers
DOI: 10.1021/ja810046q
PMID: 19449872
Source
Medline
License
Unknown

Abstract

One-third of eukaryotic proteins are integrated within membranes, as are the targets of 40% of approved drugs. However, the lack of a general means of solubilizing, stabilizing and structurally characterizing active membrane proteins has frustrated efforts to understand their mechanisms and exploit their potential value. Here we report that bilayer disks formed by phospholipids and styrene maleic anhydride copolymer preserve the functional and structural integrity of alpha-helical and beta-barrel transmembrane proteins. They form 11 nm particles that are monodispersed, biocompatible, thermostable, and water-soluble, allowing diverse membrane proteins to be simply and rapidly presented for virtually any in vitro analysis.

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