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Melatonin-induced stimulation of rat corpus epididymal epithelial cell proliferation.

Authors
  • Li, L
  • Wong, J T
  • Pang, S F
  • Shiu, S Y
Type
Published Article
Journal
Life Sciences
Publisher
Elsevier
Publication Date
Jan 01, 1999
Volume
65
Issue
10
Pages
1067–1076
Identifiers
PMID: 10499874
Source
Medline
License
Unknown

Abstract

Stimulation of rat epididymal epithelial cell proliferation by melatonin was demonstrated by thymidine incorporation and flow cytometric analyses. The stimulatory effect of melatonin was dependent on the hormone concentration and the duration of cell exposure to the hormone. Maximal stimulation of [3H]thymidine incorporation into epididymal epithelial cells by melatonin was observed at 1 x 10(-9) M 5alpha-dihydrotestosterone in medium, while lower or higher concentrations of androgen attenuated the stimulatory effect of melatonin. Interestingly, a nuclear melatonin receptor agonist (1-[3-allyl-4-oxothiazolidine-2-ylidene]-4-methyl-thiosemi-carb azone, CGP 52608) induced opposite effect on epithelial cell proliferation to that produced by melatonin. Our data suggest that melatonin-induced stimulation of rat epididymal epithelial cell proliferation is not likely to be mediated by nuclear receptor. Furthermore, sequential changes of cell cycle distribution with melatonin treatment also supports a stimulatory action of melatonin on epididymal epithelial cell proliferation.

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