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Megakaryocyte cell sorting from diosgenin-differentiated human erythroleukemia cells by sedimentation field-flow fractionation

Authors
  • Léger, D.Y.
  • Battu, S.
  • Liagre, B.
  • Beneytout, J.L.
  • Cardot, P.J.P.
Type
Published Article
Journal
Analytical Biochemistry
Publication Date
Jan 01, 2006
Volume
355
Issue
1
Pages
19–28
Identifiers
DOI: 10.1016/j.ab.2006.05.033
Source
Elsevier
Keywords
License
Unknown

Abstract

Anticancer differentiation therapy could be one strategy to stop cancer cell proliferation. Human erythroleukemia (HEL) cell line, incubated with 10 μM diosgenin, underwent megakaryocytic differentiation. Thus, the association diosgenin/HEL could be used as a model of chemically induced cellular differentiation and anticancer treatment. The goal of this work was to determine the capacity of sedimentation field-flow fractionation (SdFFF) to sort megakaryocytic differentiated cells. SdFFF cell sorting was associated with cellular characterization methods to calibrate specific elution profiles. As demonstrated by cell size measurement methods, cellular morphology, ploidy, and phenotype, we obtained an enriched, sterile, viable, and functional fraction of megakaryocytic cells. Thus, SdFFF is proposed as a routine method to prepare differentiated cells that will be further used to better understand the megakaryocytic differentiation process.

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