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Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga.

Authors
  • Mühlroth, Alice1
  • Winge, Per1
  • El Assimi, Aimen1
  • Jouhet, Juliette2
  • Maréchal, Eric2
  • Hohmann-Marriott, Martin F3
  • Vadstein, Olav3
  • Bones, Atle M4
  • 1 Department of Biology, NTNU Norwegian University of Science and Technology, 7491 Trondheim, Norway. , (Norway)
  • 2 Laboratoire de Physiologie Cellulaire Végétale, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Institut National de la Recherche Agronomique, Université Grenoble Alpes, 38000 Grenoble, France. , (France)
  • 3 Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, 7491 Trondheim, Norway. , (Norway)
  • 4 Department of Biology, NTNU Norwegian University of Science and Technology, 7491 Trondheim, Norway [email protected] , (Norway)
Type
Published Article
Journal
Plant physiology
Publication Date
Dec 01, 2017
Volume
175
Issue
4
Pages
1543–1559
Identifiers
DOI: 10.1104/pp.17.00621
PMID: 29051196
Source
Medline
Language
English
License
Unknown

Abstract

Molecular mechanisms of phosphorus (P) limitation are of great interest for understanding algal production in aquatic ecosystems. Previous studies point to P limitation-induced changes in lipid composition. As, in microalgae, the molecular mechanisms of this specific P stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and describe important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of the P substrate spectrum based on purple acid phosphatases. Increase in P trafficking displayed a rearrangement between compartments by supplying P to the chloroplast and carbon to the cytosol for lipid synthesis. We propose a novel phospholipid-recycling scheme for algae that leads to the rapid reduction of phospholipids and synthesis of the P-free lipid classes. P mobilization through membrane lipid degradation is mediated mainly by two glycerophosphoryldiester phosphodiesterases and three patatin-like phospholipases A on the transcriptome level. To compensate for low phospholipids in exponential growth, N. oceanica synthesized sulfoquinovosyldiacylglycerol and diacylglyceroltrimethylhomoserine. In this study, it was shown that an N. oceanica strain has a unique repertoire of genes that facilitate P acquisition and the degradation of phospholipids compared with other stramenopiles. The novel phospholipid-recycling scheme opens new avenues for metabolic engineering of lipid composition in algae. © 2017 American Society of Plant Biologists. All Rights Reserved.

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