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Mechanism of Assembly of Recombinant Murine Polyomavirus-Like Particles

Authors
  • Uli Schmidt
  • Rainer Rudolph
  • Gerald Böhm
Publisher
American Society for Microbiology
Publication Date
Feb 01, 2000
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

VP1 is the major viral coat protein of murine polyomavirus and can be used for the generation of virus-like particles in vitro. Here, we demonstrate that capsid assembly is an equilibrium reaction followed by oxidation of intracapsomere disulfide bonds, which are not essential for the formation of virus-like particles but enable complete particle assembly and prevent capsid dissembly.

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