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Mechanism of action of CM-55, a synthetic analogue of the antilipogenic antibiotic cerulenin.

Authors
Type
Published Article
Journal
Antimicrobial agents and chemotherapy
Publication Date
Volume
6
Issue
4
Pages
387–392
Identifiers
PMID: 4157441
Source
Medline
License
Unknown

Abstract

CM-55 is a synthetic analogue of the antibiotic cerulenin with the chemical structure of 2, 3-dodecenyl-4-oxo-dimethyl amide. This compound inhibited the growth of Saccharomyces cerevisiae ATCC 12341 and inhibited protein and lipid synthesis by 91 and 95%, respectively, at a concentration of 50 mug/ml (2.1 x 10(-4) M). The inhibition of protein synthesis was associated with the partial reduction of ribonucleic acid synthesis and leucine transport. The mechanism of inhibition of lipid synthesis was further investigated in a cell-free extract of the yeast. CM-55 inhibited the incorporation of [(14)C]acetyl Coenzyme A (CoA) into both fatty acid (FAF) and non-saponifiable fractions (NSF). However, it did not inhibit [(14)C]malonyl CoA incorporation into FAF and only slightly inhibited [(14)C]mevalonate incorporation into NSF. The activity of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) synthase was inhibited more strongly than the incorporation of [(14)C]3-hydroxy-3-methylglutaryl CoA into NSF; this could account for the CM-55 inhibition of [(14)C]acetyl CoA incorporation into NSF.

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