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Mechanical stress activates platelets at a subhemolysis level: an in vitro study.

Authors
  • Bakir, Ihsan
  • Hoylaerts, Marc F
  • Kink, Thomas
  • Foubert, Luc
  • Luyten, Peter
  • Van Kerckhoven, Soetkin
  • Leunens, Veerle
  • Bollen, Hilde
  • Reul, Helmut
  • Meyns, Bart
Type
Published Article
Journal
Artificial organs
Publication Date
Apr 01, 2007
Volume
31
Issue
4
Pages
316–323
Identifiers
PMID: 17437501
Source
Medline
License
Unknown

Abstract

A feasibility study is performed to quantify sheep platelets (PLTs) and to identify the relationship between PLT count and hemolysis as a consequence of mechanical stress. Six adult, healthy Dorset sheep have been used for in vitro blood sampling test procedures in a hemoresistometer device (HRM). In each experiment, blood of the same animal was exposed to six different shear rates. Free hemoglobin levels and PLT count for each shear rate were detected. In all animals (A-F), hemolysis increased significantly between the shear rates of 2325 and 3100/s (P < 0.05) and the mean PLT count dropped immediately (contact, low shear) 40% in the beginning, between the shear rates of 0 and 775/s (P < 0.05). PLT count increased slightly as soon as hemolysis started. At higher shear rates, hemolysis increased and PLTs reduced further. Precise counting of PLTs indicates that PLTs are consumed dramatically at very low shear (by contact) and further by applied mechanical stress when hemolysis is obvious. A repetition of these tests with human blood could indicate species differences.

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