Affordable Access

Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria

Authors
  • Yang, Krista
  • Doan, Mary T
  • Stiles, Linsey
  • Divakaruni, Ajit S
Publication Date
Sep 01, 2021
Source
eScholarship - University of California
Keywords
License
Unknown
External links

Abstract

Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to assess the potency and specificity of interventions targeting CPT-1. Strengths of the approach include ease, speed, and breadth of analysis, whereas drawbacks include loss of physiological regulation in reductionist systems and indirect assessment of CPT-1 enzymatic activity. For complete details on the use and execution of this protocol, please refer to Divakaruni et al. (2018).

Report this publication

Statistics

Seen <100 times