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Measuring Autophagy in Pancreatitis.

Authors
  • Ropolo, Alejandro1
  • Grasso, Daniel1
  • Vaccaro, Maria I2
  • 1 Pathophysiology Department, Institute of Biochemistry and Molecular Medicine (CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. , (Argentina)
  • 2 Pathophysiology Department, Institute of Biochemistry and Molecular Medicine (CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. [email protected] , (Argentina)
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2019
Volume
1880
Pages
541–554
Identifiers
DOI: 10.1007/978-1-4939-8873-0_35
PMID: 30610721
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Acute pancreatitis is one of the first pathological processes where autophagy has been described in a human tissue. Autophagy, autodigestion, and cell death are early cellular events in acute pancreatitis. Recent advances in understanding autophagy highlight its importance in pathological conditions. However, methods for monitoring autophagic activity during complex diseases, involving highly differentiated secretory cells, are complicated, and the results are sometimes misinterpreted. Here, we describe methods used to identify autophagic structures and to measure autophagic flux in cultured cell models and animal models of pancreatitis. We also briefly describe the pancreas specific autophagy mouse model that was useful to understand the actual role of autophagy in pancreatitis and to identify a novel selective autophagy pathway named zymophagy. Lastly, we describe the immunomagnetic isolation of autophagosomes and the detection of autophagy in pancreatic tissue samples derived from humans.

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