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Measurement of serum IgG4 levels by a competitive immunoenzymatic assay with monoclonal antibodies.

Authors
Type
Published Article
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
74
Issue
1
Pages
151–162
Identifiers
PMID: 6438233
Source
Medline
License
Unknown

Abstract

A competitive indirect ELISA is described for the measurement of IgG4 levels. It uses a monoclonal anti-subclass and antibody and purified monoclonal IgG4 as standards. This method is sensitive and reproducible and more accurate than hemagglutination inhibition and radial immunodiffusion. Serum IgG4 levels in 173 normal adults were less than 0.01-2.1 mg/ml (mean 0.30 mg/ml) in women and less than 0.01-1.87 mg/ml (mean 0.465 mg/ml) in men.

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