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Measurement of Indolebutyric Acid in Plant Tissues by Isotope Dilution Gas Chromatography-Mass Spectrometry Analysis 1

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Abstract

An internal standard, [13C][indole-2]-indole-3-butyric acid, was synthesized from indole-2[13C] and was shown to be effective for the quantitative determination of indole-3-butyric acid from plant tissue. When this standard was used along with [13C6]indole-3-acetic acid, both indolic auxins could be quantified from the same tobacco (Nicotiana tabacam) leaf sample by isotope dilution analysis using selected ion monitoring gas chromatography-mass spectrometry for detection.

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