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Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR.

Authors
Type
Published Article
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
69
Issue
2
Pages
414–416
Identifiers
PMID: 17250913
Source
Medline

Abstract

Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.

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