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Matrix-based yeast two-hybrid screen strategies and comparison of systems.

Authors
Type
Published Article
Journal
Methods in Molecular Biology
1064-3745
Publication Date
Volume
812
Pages
1–20
Identifiers
DOI: 10.1007/978-1-61779-455-1_1
PMID: 22218851
Source
Medline

Abstract

Today, matrix-based screens are used primarily for smaller and medium-size clone collections in combination with automation and cloning techniques that allow for reliable and fast interaction screening. Matrix-based yeast two-hybrid screens are an alternative to library-based screens. However, intermediary forms are possible too and we compare both strategies, including a detailed discussion of matrix-based screens. Recent improvement of matrix screens (also called array screens) uses various pooling strategies as well as novel vectors that increase their efficiency while decreasing false-negative rates and increasing reliability.

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