Affordable Access

Matrix-assisted laser desorption of peptides and proteins on a quadrupole ion trap mass spectrometer.

Authors
  • Jonscher, K
  • Currie, G
  • McCormack, A L
  • Yates, J R 3rd
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Jan 01, 1993
Volume
7
Issue
1
Pages
20–26
Identifiers
PMID: 8428025
Source
Medline
License
Unknown

Abstract

The use of ultraviolet matrix-assisted laser desorption (MALD) to ionize peptides and proteins for analysis in a quadrupole ion trap is described. An ion source was modified to accommodate a fiber optic to transmit laser radiation from a nitrogen laser (337 nm) to the tip of the sample probe containing peptide or protein samples in a matrix of 2,5-dihydroxybenzoic acid (DHB) or 3,4-dimethoxy-4-hydroxy-cinnamic acid. Detection limits are demonstrated with 10 fmol of sperm-whale myoglobin. The dimer of sperm-whale myoglobin was also observed at m/z 34,430. A comparison is made of the tandem mass spectrum of (MS/MS) of human angiotensin I desorbed by MALD to that of the peptide desorbed by liquid secondary-ion mass spectrometry. Both spectra were found to contain abundant structural information.

Report this publication

Statistics

Seen <100 times