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Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.

Authors
  • Kirpekar, F
  • Nordhoff, E
  • Kristiansen, K
  • Roepstorff, P
  • Lezius, A
  • Hahner, S
  • Karas, M
  • Hillenkamp, F
Type
Published Article
Journal
Nucleic acids research
Publication Date
Sep 25, 1994
Volume
22
Issue
19
Pages
3866–3870
Identifiers
PMID: 7524029
Source
Medline
License
Unknown

Abstract

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

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