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A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate-polyacrylamide electrophoresis gels.

Authors
  • Jin, Li-Tai
  • Hwang, Sun-Young
  • Yoo, Gyurng-Soo
  • Choi, Jung-Kap
Type
Published Article
Journal
Proteomics
Publication Date
Apr 01, 2006
Volume
6
Issue
8
Pages
2334–2337
Identifiers
PMID: 16493709
Source
Medline
License
Unknown

Abstract

A quick, sensitive, and MALDI-TOF MS compatible silver staining method, namely Eriochrome black T (EBT)-silver method, is described. The method can detect 0.05-0.2 ng protein within 60 min in SDS-PAGE gels. EBT dye was used as a silver ion sensitizer having reducing power for silver ions.

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