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Mass Spectrometry Advances and Perspectives for the Characterization of Emerging Adoptive Cell Therapies

Authors
  • Lombard-Banek, Camille1
  • Schiel, John E.1
  • 1 Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA
Type
Published Article
Journal
Molecules
Publisher
MDPI AG
Publication Date
Mar 19, 2020
Volume
25
Issue
6
Identifiers
DOI: 10.3390/molecules25061396
PMID: 32204371
PMCID: PMC7144572
Source
PubMed Central
Keywords
License
Green

Abstract

Adoptive cell therapy is an emerging anti-cancer modality, whereby the patient’s own immune cells are engineered to express T-cell receptor (TCR) or chimeric antigen receptor (CAR). CAR-T cell therapies have advanced the furthest, with recent approvals of two treatments by the Food and Drug Administration of Kymriah (trisagenlecleucel) and Yescarta (axicabtagene ciloleucel). Recent developments in proteomic analysis by mass spectrometry (MS) make this technology uniquely suited to enable the comprehensive identification and quantification of the relevant biochemical architecture of CAR-T cell therapies and fulfill current unmet needs for CAR-T product knowledge. These advances include improved sample preparation methods, enhanced separation technologies, and extension of MS-based proteomic to single cells. Innovative technologies such as proteomic analysis of raw material quality attributes (MQA) and final product quality attributes (PQA) may provide insights that could ultimately fuel development strategies and lead to broad implementation.

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