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Mass spectrometric identification of RNA binding proteins from dried EMSA gels.

Authors
  • Stenger, Dominique
  • Gruissem, Wilhelm
  • Baginsky, Sacha
Type
Published Article
Journal
Journal of proteome research
Publication Date
Jan 01, 2004
Volume
3
Issue
3
Pages
662–664
Identifiers
PMID: 15253451
Source
Medline
License
Unknown

Abstract

Electrophoretic mobility shift assays (EMSA) are commonly employed for the analysis of nucleic acid/ protein interactions with a native gel system. Here, we report a method to identify RNA binding proteins from a dried EMSA gel by mass spectrometry following autoradiography. Compared to wet gel exposure, our approach resulted in an improved protein identification sensitivity and RNA/protein complex isolation accuracy. The method described here is useful for the large scale characterization of RNA- or DNA-protein complexes.

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