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Mass Spectrometric Analysis of Acidic Exo-Oligosaccharides of Root Nodule Bacterium Rhizobium leguminosarum bv. viciae VF39

Authors
  • Kanapina, A. S.1
  • Marchenkov, V. V.2
  • Surin, A. K.3
  • Ivashina, T. V.1
  • 1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Russia , Pushchino (Russia)
  • 2 Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290, Russia , Pushchino (Russia)
  • 3 Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia , Pushchino (Russia)
Type
Published Article
Journal
Microbiology
Publisher
Pleiades Publishing
Publication Date
Sep 01, 2020
Volume
89
Issue
5
Pages
520–531
Identifiers
DOI: 10.1134/S0026261720050112
Source
Springer Nature
Keywords
License
Yellow

Abstract

Abstract—The structures of exo-oligosaccharides produced by a symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum bv. viciae VF39 were determined using the methods of mass spectrometry and HCD fragmentation of ions. Exo-oligosaccharides were shown to be monomers, dimers and trimers of an octasaccharide repeating unit of the high-molecular-weight acidic exopolysaccharide. The major fraction was represented by octasaccharide monomers with two glucuronic acid residues at the reducing end. Heptasaccharides corresponding to an octasaccharide unit without GlcA-c or Glc-b residues in the main chain were also found in the culture liquid. Heterogeneity of exo-oligosaccharides according to the content of O-acetyl and hydroxybutanoyl substituents was revealed.

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