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Marine bacterial DNase curtails virulence and disrupts biofilms of Candida albicans and non-albicans Candida species.

Authors
  • Farisa Banu, Sanaulla1
  • Thamotharan, Subbiah2
  • Gowrishankar, Shanmugaraj3
  • Karutha Pandian, Shunmugiah3
  • Nithyanand, Paramasivam1
  • 1 Biofilm Biology Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India. , (India)
  • 2 Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India. , (India)
  • 3 Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India. , (India)
Type
Published Article
Journal
Biofouling
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 01, 2019
Volume
35
Issue
9
Pages
975–985
Identifiers
DOI: 10.1080/08927014.2019.1680650
PMID: 31779493
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Candida is one of the most prevalent fungal pathogens in clinical settings which form antibiotic-resistant biofilms on biomedical devices. Hence, there is a need for non-antimicrobial alternatives to combat these infections. The present study investigates the anti-biofilm effect of marine bacterial DNase by targeting the eDNA present in the biofilms of Candida spp. A strain of Vibrio alginolyticus (AMSII) which showed enhanced DNase activity was isolated from marine sediment. Treatment of young and mature Candida biofilms with purified marine bacterial DNase (MBD) caused a 60-80% reduction in biofilm biomass, similar to treatment with DNase I from Bovine pancreas. Scanning electron microscopy showed that MBD significantly reduced the formation of biofilms on urinary catheters and more importantly prevented the virulent yeast to hyphae dimorphic switch in C. albicans. The present study identified a potential non-antibiotic alternative therapy to eradicate Candida biofilms and can be used to develop enzyme fabricated antifouling indwelling medical devices.

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