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Mapping signal transduction pathways by phage display.

Authors
  • Zozulya, S
  • Lioubin, M
  • Hill, R J
  • Abram, C
  • Gishizky, M L
Type
Published Article
Journal
Nature biotechnology
Publication Date
Dec 01, 1999
Volume
17
Issue
12
Pages
1193–1198
Identifiers
PMID: 10585717
Source
Medline
License
Unknown

Abstract

Rapid identification of proteins that interact with a novel gene product is an important element of functional genomics. Here we describe a phage display-based technique for interaction screening of complex cDNA libraries using proteins or synthetic peptides as baits. Starting with the epidermal growth factor receptor (EGFR) cytoplasmic tail, we identified known protein interactions that link EGFR to the Ras/MAP kinase signal transduction cascade and several novel interactions. This approach can be used as a rapid and efficient tool for elucidating protein networks and mapping intracellular signal transduction pathways.

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