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A map of the interactome network of the metazoan C. elegans.

Authors
  • Li, Siming1
  • Armstrong, Christopher M
  • Bertin, Nicolas
  • Ge, Hui
  • Milstein, Stuart
  • Boxem, Mike
  • Vidalain, Pierre-Olivier
  • Han, Jing-Dong J
  • Chesneau, Alban
  • Hao, Tong
  • Goldberg, Debra S
  • Li, Ning
  • Martinez, Monica
  • Rual, Jean-François
  • Lamesch, Philippe
  • Xu, Lai
  • Tewari, Muneesh
  • Wong, Sharyl L
  • Zhang, Lan V
  • Berriz, Gabriel F
  • And 28 more
  • 1 Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
Type
Published Article
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
Jan 23, 2004
Volume
303
Issue
5657
Pages
540–543
Identifiers
PMID: 14704431
Source
Medline
Language
English
License
Unknown

Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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