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Mammalian U2 snRNP has a sequence-specific RNA-binding activity.

Authors
Type
Published Article
Journal
Genes & Development
0890-9369
Publisher
Cold Spring Harbor Laboratory
Publication Date
Volume
3
Issue
10
Pages
1562–1571
Identifiers
PMID: 2612905
Source
Medline
License
Unknown

Abstract

The RNA branch formed during pre-mRNA splicing occurs at a wide variety of sequences (branch sites) in different mammalian pre-mRNAs. U2 small nuclear ribonucleoprotein (snRNP) binds to the pre-mRNA branch site following the interaction of a protein, U2AF, with the 3' splice site/polypyrimidine tract. Here we show that despite the variability of mammalian branch sites, U2 snRNP has a sequence-specific RNA-binding activity. Thus, RNA branch formation is regulated by two sequence-specific interactions: U2AF with the 3' splice site/polypyrimidine tract, and U2 snRNP with the branch site. The affinity of the branch site for U2 snRNP affects the efficiency of spliceosome assembly and splicing.

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